Disruption of ζ‑Carotene Desaturase Protein ALE1 Leads to Chloroplast Developmental Defects and Seedling Lethality

Published on 2019-10-09T17:03:46Z (GMT) by
ζ-carotene desaturase (ZDS) is a key enzyme in carotenoid biosynthesis and plays an important role in plant photosynthesis. We characterized an albino leaf-color mutant obtained from ethyl methanesulfonate treatment: albino and seedling lethality 1 (<i>ale1</i>). The material contains a chloroplast thylakoid defect where photosynthetic pigments declined and reactive oxygen species accumulated resulting in <i>ale1</i> death within 3 weeks. Positional cloning and sequencing revealed that there was a single base substitution in <i>ALE1</i>, which encoded a ZDS involved in carotenoid biosynthesis. RNAi and complementation tests confirmed the identity of <i>ALE1</i>. Subcellular localization showed that the ALE1 protein is localized in the chloroplast. Expression analysis indicated that the genes involved in chlorophyll and carotenoid biosynthesis were downregulated. We conclude that <i>ALE1</i> plays an important role in chloroplast and plant growth in rice.

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Fang, Yunxia; Hou, Linlin; Zhang, Xiaoqin; Pan, Jiangjie; Ren, Deyong; Zeng, Dali; et al. (2019): Disruption of ζ‑Carotene Desaturase Protein

ALE1 Leads to Chloroplast Developmental Defects and Seedling Lethality. ACS Publications. Collection. https://doi.org/10.1021/acs.jafc.9b05051