Discovery of a Novel Nitric Oxide Binding Protein
and Nitric-Oxide-Responsive Signaling Pathway in Pseudomonas
aeruginosa
Version 3 2017-06-16, 20:32
Version 2 2017-03-16, 18:26
Version 1 2017-03-16, 18:22
Posted on 2017-06-16 - 20:32
Nitric
oxide (NO) is a radical diatomic gas molecule that, at low concentrations,
plays important signaling roles in both eukaryotes and bacteria. In
recent years, it has become evident that bacteria respond to low levels
of NO in order to modulate their group behavior. Many bacteria respond
via NO ligation to a well-established NO sensor called H-NOX (heme-nitric
oxide/oxygen binding domain). Many others, such as Pseudomonas aeruginosa, lack an annotated hnoX gene in their genome yet are able to respond to low
levels of NO to disperse their biofilms. This suggests the existence
of a previously uncharacterized NO sensor. In this study, we describe
the discovery of a novel nitric oxide binding protein (NosP; NO-sensing
protein), which is much more widely conserved in bacteria than H-NOX,
as well as a novel NO-responsive pathway in P. aeruginosa. We demonstrate that biofilms of a P. aeruginosa mutant lacking components of the NosP pathway lose the ability to
disperse in response to NO. Upon cloning, expressing, and purifying
NosP, we find it binds heme and ligates to NO with a dissociation
rate constant that is comparable to that of other well-established
NO-sensing proteins. Moreover, we show that NO-bound NosP is able
to regulate the phosphorelay activity of a hybrid histidine kinase
that is involved in biofilm regulation in P. aeruginosa. Thus, here, we present evidence of a novel NO-responsive pathway
that regulates biofilm in P. aeruginosa.
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Hossain, Sajjad; Boon, Elizabeth M. (2017). Discovery of a Novel Nitric Oxide Binding Protein
and Nitric-Oxide-Responsive Signaling Pathway in Pseudomonas
aeruginosa. ACS Publications. Collection. https://doi.org/10.1021/acsinfecdis.7b00027
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AUTHORS (2)
SH
Sajjad Hossain
EB
Elizabeth M. Boon
KEYWORDS
NO-sensing proteinshistidine kinasebiofilm regulationNO-bound NosPphosphorelay activityhnoX geneH-NOXNitric-Oxide-Responsive Signaling PathwayNovel Nitric Oxide Binding Proteinnovel NO-responsive pathwayNosP pathwayMany othersMany bacteriaPseudomonas aeruginosanovel nitric oxide binding proteinPseudomonas aeruginosa Nitric oxidegas moleculegroup behaviordissociation rate