Development of MicroRNA-146a-Enriched Stem Cell Secretome
for Wound-Healing Applications
Posted on 2019-08-23 - 21:15
Secretome-based
therapies have the potential to become the next
generation of viable therapeutic wound repair treatments. However,
precise strategies aimed to refine and control the secretome composition
are necessary to enhance its therapeutic efficacy and facilitate clinical
translation. In this study, we aim to accomplish this by transfecting
human adipose-derived stem cells (hASCs) with microRNA-146a, which
is a potent regulator of angiogenesis and inflammation. The secretome
composition obtained from the transfected hASCs (secretome146a) was characterized and compared to nontransfected hASCs secretome
to evaluate changes in angiogenic and anti-inflammatory growth factor,
cytokine, and miRNA content. In vitro proliferation, migration, and
tubular morphogenesis assays using human umbilical vein endothelial
cells (HUVECs) were completed to monitor the proangiogenic efficacy
of the secretome146a. Finally, the anti-inflammatory efficacy
of the secretome146a was assessed using HUVECs that were
activated to an inflammatory state by IL-1β. The resulting HUVEC
gene expression and protein activity of key inflammatory mediators
were evaluated before and after secretome treatment. Overall, the
secretome146a contained a greater array and concentration
of therapeutic paracrine molecules, which translated into a superior
angiogenic and anti-inflammatory efficacy. Therefore, this represents
a promising strategy to produce therapeutic secretome for the promotion
of wound repair processes.
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Waters, Renae; Subham, Siddharth; Pacelli, Settimio; Modaresi, Saman; Chakravarti, Aparna R.; Paul, Arghya (2019). Development of MicroRNA-146a-Enriched Stem Cell Secretome
for Wound-Healing Applications. ACS Publications. Collection. https://doi.org/10.1021/acs.molpharmaceut.9b00639
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AUTHORS (6)
RW
Renae Waters
SS
Siddharth Subham
SP
Settimio Pacelli
SM
Saman Modaresi
AC
Aparna R. Chakravarti
AP
Arghya Paul
CATEGORIES
- Biophysics
- Biochemistry
- Space Science
- Microbiology
- Cell Biology
- Molecular Biology
- Pharmacology
- Biotechnology
- Environmental Sciences not elsewhere classified
- Chemical Sciences not elsewhere classified
- Immunology
- Biological Sciences not elsewhere classified
- Developmental Biology
- Cancer
- Hematology
- Infectious Diseases