Decellularized Tissue Matrix Enhances Self-Assembly
of Islet Organoids from Pluripotent Stem Cell Differentiation
Posted on 2020-06-11 - 21:43
Regenerating
human islet organoids from stem cells remains a significant
challenge because of our limited knowledge on cues essential for developing
the endocrine organoids in vitro. In this study, we discovered that
a natural material prepared from a decellularized rat pancreatic extracellular
matrix (dpECM) induces the self-assembly of human islet organoids
during induced pluripotent stem cell (iPSC) pancreatic differentiation.
For the first time, we demonstrated that the iPSC-derived islet organoids
formed in the presence of the dpECM are capable of glucose-responsive
secretion of both insulin and glucagon, two major hormones that maintain
blood glucose homeostasis. The characterization of the organoids revealed
that the organoids consisted of all major endocrine cell types, including
α, β, δ, and pancreatic polypeptide cells, that
were assembled into a tissue architecture similar to that of human
islets. The exposure of iPSCs to the dpECM during differentiation
resulted in considerably elevated expression of key pancreatic transcription
factors such as PDX-1, MAFA, and NKX6.1 and the production of all
major hormones, including insulin, glucagon, somatostatin, and pancreatic
polypeptide from stem cell-derived organoids. This study highlights
the importance of natural, bioactive biomaterials for building microenvironments
crucial to regenerating islet organoids from stem cells.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Bi, Huanjing; Karanth, Soujanya S.; Ye, Kaiming; Stein, Roland; Jin, Sha (2020). Decellularized Tissue Matrix Enhances Self-Assembly
of Islet Organoids from Pluripotent Stem Cell Differentiation. ACS Publications. Collection. https://doi.org/10.1021/acsbiomaterials.0c00088
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (5)
HB
Huanjing Bi
SK
Soujanya S. Karanth
KY
Kaiming Ye
RS
Roland Stein
SJ
Sha Jin