Controlled Reduction of Photobleaching in DNA Origami–Gold
Nanoparticle Hybrids
Posted on 2014-05-14 - 00:00
The amount of information obtainable
from a fluorescence-based
measurement is limited by photobleaching: Irreversible photochemical
reactions either render the molecules nonfluorescent or shift their
absorption and/or emission spectra outside the working range. Photobleaching
is evidenced as a decrease of fluorescence intensity with time, or
in the case of single molecule measurements, as an abrupt, single-step
interruption of the fluorescence emission that determines the end
of the experiment. Reducing photobleaching is central for improving
fluorescence (functional) imaging, single molecule tracking, and fluorescence-based
biosensors and assays. In this single molecule study, we use DNA self-assembly
to produce hybrid nanostructures containing individual fluorophores
and gold nanoparticles at a controlled separation distance of 8.5
nm. By changing the nanoparticles’ size we are able to systematically
increase the mean number of photons emitted by the fluorophores before
photobleaching.
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Pellegrotti, Jesica
V.; Acuna, Guillermo P.; Puchkova, Anastasiya; Holzmeister, Phil; Gietl, Andreas; Lalkens, Birka; et al. (2016). Controlled Reduction of Photobleaching in DNA Origami–Gold
Nanoparticle Hybrids. ACS Publications. Collection. https://doi.org/10.1021/nl500841n
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AUTHORS (8)
JP
Jesica
V. Pellegrotti
GA
Guillermo P. Acuna
AP
Anastasiya Puchkova
PH
Phil Holzmeister
AG
Andreas Gietl
BL
Birka Lalkens
FS
Fernando D. Stefani
PT
Philip Tinnefeld