Comparative Analysis of Two Flavonol Synthases from
Different-Colored Onions Provides Insight into Flavonoid Biosynthesis
Version 2 2017-06-23, 02:48
Version 1 2017-06-22, 14:04
Posted on 2017-06-23 - 02:48
We isolated cDNAs
encoding flavonol synthase (FLS) from the red
onion “H6” (AcFLS-H6) and the yellow
onion “Hwangryongball” (AcFLS-HRB).
We found three amino acid variations between the two sequences. Kinetic
analysis with recombinant proteins revealed that AcFLS-HRB exhibited
approximately 2-fold higher catalytic efficiencies than AcFLS-H6 for
dihydroflavonol substrates and that both proteins preferred dihydroquercetin
to dihydrokaempferol. The expression patterns of flavonoid biosynthesis
genes corresponded to the accumulation patterns of flavonoid aglycones
in both onions. Whereas the other flavonoid biosynthesis genes were
weakly expressed in the HRB sheath compared to that of H6, the expression
of FLS was similar in both onions. This relatively
enhanced FLS expression, along with the higher activity
of AcFLS-HRB, could increase the quercetin production in the HRB sheath.
The quercetin content was approximately 12-fold higher than the cyanidin
content in the H6 sheath, suggesting that FLS has priority in the
competition between FLS and dihydroflavonol 4-reductase (DFR) for
their substrate dihydroquercetin.
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Park, Sangkyu; Kim, Da-Hye; Lee, Jong-Yeol; Ha, Sun-Hwa; Lim, Sun-Hyung (2017). Comparative Analysis of Two Flavonol Synthases from
Different-Colored Onions Provides Insight into Flavonoid Biosynthesis. ACS Publications. Collection. https://doi.org/10.1021/acs.jafc.7b01036
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AUTHORS (5)
SP
Sangkyu Park
DK
Da-Hye Kim
JL
Jong-Yeol Lee
SH
Sun-Hwa Ha
SL
Sun-Hyung Lim