Click Chemistry to Construct Fluorescent
Oligonucleotides for DNA Sequencing

Ju, Jingyue

Click Chemistry to Construct Fluorescent Oligonucleotides for DNA Sequencing

Posted on 2002-12-21 - 00:00

“Click chemistry” 1,3-dipolar cycloaddition between alkynyl 6-carboxyfluorescein (FAM) and azido-labeled single-stranded (ss) DNA was carried out under aqueous conditions to produce FAM-labeled ssDNA in quantitative yield. The FAM-labeled ssDNA was successfully used as a primer to produce DNA sequencing products with single-base resolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence detection.

CITE THIS COLLECTION

Seo, Tae Seok; Li, Zengmin; Ruparel, Hameer; Ju, Jingyue (2016). Click Chemistry to Construct Fluorescent Oligonucleotides for DNA Sequencing. ACS Publications. Collection. https://doi.org/10.1021/jo026615r

https://doi.org/10.1021/jo026615r

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