Birth of a Photosynthetic Chassis: A MoClo Toolkit
Enabling Synthetic Biology in the Microalga Chlamydomonas
reinhardtii
Version 2 2018-09-06, 16:19
Version 1 2018-09-05, 20:05
Posted on 2018-09-06 - 16:19
Microalgae
are regarded as promising organisms to develop innovative
concepts based on their photosynthetic capacity that offers more sustainable
production than heterotrophic hosts. However, to realize their potential
as green cell factories, a major challenge is to make microalgae easier
to engineer. A promising approach for rapid and predictable genetic
manipulation is to use standardized synthetic biology tools and workflows.
To this end we have developed a Modular Cloning toolkit for the green
microalga Chlamydomonas reinhardtii. It is based
on Golden Gate cloning with standard syntax, and comprises 119 openly
distributed genetic parts, most of which have been functionally validated
in several strains. It contains promoters, UTRs, terminators, tags,
reporters, antibiotic resistance genes, and introns cloned in various
positions to allow maximum modularity. The toolkit enables rapid building
of engineered cells for both fundamental research and algal biotechnology.
This work will make Chlamydomonas the next chassis
for sustainable synthetic biology.
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Crozet, Pierre; Navarro, Francisco J.; Willmund, Felix; Mehrshahi, Payam; Bakowski, Kamil; Lauersen, Kyle J.; et al. (2018). Birth of a Photosynthetic Chassis: A MoClo Toolkit
Enabling Synthetic Biology in the Microalga Chlamydomonas
reinhardtii. ACS Publications. Collection. https://doi.org/10.1021/acssynbio.8b00251
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AUTHORS (33)
PC
Pierre Crozet
FN
Francisco J. Navarro
FW
Felix Willmund
PM
Payam Mehrshahi
KB
Kamil Bakowski
KL
Kyle J. Lauersen
MP
Maria-Esther Pérez-Pérez
PA
Pascaline Auroy
AG
Aleix Gorchs Rovira
SS
Susana Sauret-Gueto
JN
Justus Niemeyer
BS
Benjamin Spaniol
JT
Jasmine Theis
RT
Raphael Trösch
LW
Lisa-Desiree Westrich
KV
Konstantinos Vavitsas
TB
Thomas Baier
WH
Wolfgang Hübner
Fd
Felix de Carpentier
MC
Mathieu Cassarini