Biomimetic, Multiresponsive, and Self-Healing Lactose-Modified
Chitosan (CTL)-Based Gels Formed via Competitor-Assisted Mechanism
Posted on 2019-09-13 - 18:35
The
present paper describes an original method to form under physiological
conditions homogeneous lactose-modified chitosan (CTL) gels avoiding
syneresis. Specifically, combination of boric acidi.e., the
cross-linkerand mannitoli.e., a polyol competitor
for boron bindingwere exploited to reduce the very fast kinetics
of CTL/boron self-assembly. Resulting gels were homogeneous as proved
by scattering analyses. An in-depth rheological characterization was
undertaken to identify the correct mannitol-to-boron ratio at which
gels showed homogeneity without weakening. Stress sweep and frequency
sweep tests were performed to investigate the viscoelastic properties
of these dynamic networks, highlighting a marked strain-hardening
behavior, which is pivotal in native tissues. Notably, herein we report
for the first time that CTL–boric acid gels are multiresponsive
systems, whose mechanics can be tailored by different stimuli such
as the presence of small molecules like glucose. Moreover, we demonstrate
that these networks spontaneously self-heal after breakage. The biocompatibility
of such gels was studied under 2D and 3D conditions toward three different
cell models, namely, pig primary chondrocytes, human Dental Pulp Stem
Cells (hDPSCs), and mouse fibroblasts. Giving the peculiar mechanical
performance of selected systems and considering the well-known bioactivity
of the chitosan derivative, CTL–boric acid networks are promising
candidates as multiresponsive gels to be used in the field of tissue
engineering, especially for articular cartilage regeneration.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Furlani, Franco; Sacco, Pasquale; Cok, Michela; de Marzo, Gaia; Marsich, Eleonora; Paoletti, Sergio; et al. (2019). Biomimetic, Multiresponsive, and Self-Healing Lactose-Modified
Chitosan (CTL)-Based Gels Formed via Competitor-Assisted Mechanism. ACS Publications. Collection. https://doi.org/10.1021/acsbiomaterials.9b01256
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (7)
FF
Franco Furlani
PS
Pasquale Sacco
MC
Michela Cok
Gd
Gaia de Marzo
EM
Eleonora Marsich
SP
Sergio Paoletti
ID
Ivan Donati
KEYWORDS
tissue engineeringarticular cartilage regenerationmultiresponsive systemsstrain-hardening behaviorDental Pulp Stem Cells2 Dacidpolyol competitorcell modelsSelf-Healing Lactose-Modified Chitosanlactose-modified chitosanCompetitor-Assisted Mechanismrheological characterizationmannitol-to-boron ratioCTL3 D conditionsfrequency sweep testsmouse fibroblastsStress sweep