Binding of Bacteria to Poly(N‑isopropylacrylamide)
Modified with Vancomycin: Comparison of Behavior of Linear and Highly
Branched Polymers
Version 2 2017-08-23, 04:22
Version 1 2017-08-18, 14:43
Posted on 2017-08-23 - 04:22
The
behavior of a linear copolymer of N-isopropylacrylamide
with pendant vancomycin functionality was compared to an analogous
highly branched copolymer with vancomycin functionality at the chain
ends. Highly branched poly(N-isopropylacrylamide)
modified with vancomycin (HB-PNIPAM-van) was synthesized by functionalization
of the HB-PNIPAM, prepared using reversible addition–fragmentation
chain transfer polymerization. Linear PNIPAM with pendant vancomycin
functionality (L-PNIPAM-van) was synthesized by functionalization
of poly(N-isopropylacrylamide-co-vinyl benzoic acid). HB-PNIPAM-van aggregated S. aureus effectively, whereas the L-PNIPAM-van polymer did not. It was found
that when the HB-PNIPAM-van was incubated with S. aureus the resultant phase transition provided an increase in the intensity
of fluorescence of a solvatochromic dye, nile red, added to the system.
In contrast, a significantly lower increase in fluorescence intensity
was obtained when L-PNIPAM-van was incubated with S. aureus. These data showed that the degree of desolvation of HB-PNIPAM-van
was much greater than the desolvation of the linear version. Using
microcalorimetry, it was shown that there were no significant differences
in the affinities of the polymer ligands for d-Ala-d-Ala and therefore differences in the interactions with bacteria
were associated with changes in the probability of access of the polymer
bound ligands to the d-Ala-d-Ala dipeptide. The
data support the hypothesis that generation of polymer systems that
respond to cellular targets, for applications such as cell targeting,
detection of pathogens etc., requires the use of branched polymers
with ligands situated at the chain ends.
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Teratanatorn, Pavintorn; Hoskins, Richard; Swift, Thomas; W. Ian Douglas, C.; Shepherd, Joanna; Rimmer, Stephen (2017). Binding of Bacteria to Poly(N‑isopropylacrylamide)
Modified with Vancomycin: Comparison of Behavior of Linear and Highly
Branched Polymers. ACS Publications. Collection. https://doi.org/10.1021/acs.biomac.7b00800
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AUTHORS (6)
PT
Pavintorn Teratanatorn
RH
Richard Hoskins
TS
Thomas Swift
CW
C. W. Ian Douglas
JS
Joanna Shepherd
SR
Stephen Rimmer