Assessment of a Silicon-Photomultiplier-Based Platform
for the Measurement of Intracellular Calcium Dynamics with Targeted
Aequorin
Posted on 2020-08-05 - 19:04
Ca2+ is among the most important intracellular second
messengers participating in a plethora of biological processes, and
the measurement of Ca2+ fluctuations is significant in
the phenomenology of the underlying processes. Aequorin-based Ca2+ probes represent an invaluable tool for reliable measurement
of Ca2+ concentrations and dynamics in different subcellular
compartments. However, their use is limited due to the lack on the
market of ready-to-use, cost-effective, and portable devices for the
detection and readout of the low-intensity bioluminescence signal
produced by these probes. Silicon photomultipliers (SiPMs) are rapidly
evolving solid-state sensors for low light detection, with single
photon sensitivity and photon number resolving capability, featuring
low cost, low voltage, and compact format. Thus, they may represent
the sensors of choice for the development of such devices and, more
in general, of a new generation of multipurpose bioluminescence detectors
suitable for cell biology studies. Ideally, a detector customized
for these purposes must combine high dynamic range with high fidelity
in reconstructing the light intensity signal temporal profile. In
this article, the ability to perform aequorin-based intracellular
Ca2+ measurements using a multipurpose, low-cost setup
exploiting SiPMs as the sensors is demonstrated. SiPMs turn out to
assure performances comparable to those exhibited by a custom-designed
photomultiplier tube-based aequorinometer. Moreover, the flexibility
of SiPM-based devices might pave the way toward routinely and wide
scale application of innovative biophysical protocols.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Ruffinatti, Federico
Alessandro; Lomazzi, Samuela; Nardo, Luca; Santoro, Romualdo; Martemiyanov, Alexander; Dionisi, Marianna; et al. (2020). Assessment of a Silicon-Photomultiplier-Based Platform
for the Measurement of Intracellular Calcium Dynamics with Targeted
Aequorin. ACS Publications. Collection. https://doi.org/10.1021/acssensors.0c00277
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (11)
FR
Federico
Alessandro Ruffinatti
SL
Samuela Lomazzi
LN
Luca Nardo
RS
Romualdo Santoro
AM
Alexander Martemiyanov
MD
Marianna Dionisi
LT
Laura Tapella
AG
Armando A. Genazzani
DL
Dmitry Lim
CD
Carla Distasi
MC
Massimo Caccia
KEYWORDS
probephoton numberSilicon-Photomultiplier-Based Platformlow-intensity bioluminescence signalmeasurementsensorsubcellular compartmentsphoton sensitivitycell biology studiesscale applicationbioluminescence detectorssilicon photomultipliersIntracellular Calcium Dynamicsphotomultiplier tube-based aequorin...Targeted AequorinSiPM-based deviceslight detectionlight intensity signalaequorin-based intracellular