Arraying of Single Cells for Quantitative High Throughput
Laser Ablation ICP-TOF-MS
Posted on 2019-08-27 - 20:20
Arraying
of single cells for mass spectrometric analysis is a considerable
bioanalytical challenge. In this study, we employ a novel single cell
arraying technology for quantitative analysis and isotopic fingerprinting
by laser ablation inductively coupled plasma time-of-flight mass spectrometry
(LA-ICP-TOF-MS). The single cell arraying approach is based on a piezo-acoustic
microarrayer with software for automated optical detection of cells
within the piezo dispense capillary (PDC) prior to arraying. Using
optimized parameters, single cell occupancy of >99%, high throughput
(up to 550 cells per hour), and a high cell recovery of >66% is
achieved.
LA-ICP-TOF-MS is employed to detect naturally occurring isotopes in
the whole mass range as fingerprints of individual cells. Moreover,
precise quantitative determination of metal-containing cell dyes is
possible down to contents of ∼100 ag using calibration standards
which were produced using the same arrayer.
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Löhr, Konrad; Borovinskaya, Olga; Tourniaire, Guilhem; Panne, Ulrich; Jakubowski, Norbert (2019). Arraying of Single Cells for Quantitative High Throughput
Laser Ablation ICP-TOF-MS. ACS Publications. Collection. https://doi.org/10.1021/acs.analchem.9b00198