Amination of a
Green Solvent via Immobilized Biocatalysis
for the Synthesis of Nemtabrutinib
Posted on 2023-05-26 - 12:20
Enzymes
are capable of unique and selective transformations that
can enable efficient chemical production. While many industrial processes
have been developed using free enzymes in aqueous solutions, immobilizing
enzymes on a solid support can offer considerable advantages, including
improved reaction efficiency, enzyme stability, the ability to perform
reactions in non-aqueous media, and simplified separation of the product
from the enzyme. Herein, we describe the development of a biocatalytic
transaminase reaction of Cyrene (2) utilizing an immobilized,
evolved transaminase enzyme in an organic solvent to provide amine
intermediate 3a en route to Bruton’s tyrosine
kinase (BTK) inhibitor nemtabrutinib. Enzyme immobilization is critical
to facile isolation of the water-soluble product. Improved reaction
kinetics and diastereoselectivity were achieved by bridging directed
enzyme evolution with the selection of an optimal reaction solvent
and solid support for immobilization, enabling a unified solvent system
and direct isolation of 3a as a crystalline salt with dr > 50:1.
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Prier, Christopher
K.; Camacho Soto, Karla; Forstater, Jacob H.; Kuhl, Nadine; Kuethe, Jeffrey T.; Cheung-Lee, Wai Ling; et al. (2023). Amination of a
Green Solvent via Immobilized Biocatalysis
for the Synthesis of Nemtabrutinib. ACS Publications. Collection. https://doi.org/10.1021/acscatal.3c00941