Alterations in
Lipid Profile of the Aging Kidney Identified
by MALDI Imaging Mass Spectrometry
Version 2 2019-06-19, 13:08
Version 1 2019-06-19, 13:06
Posted on 2019-06-19 - 13:08
During
aging, the kidney undergoes functional and physiological
changes that are closely affiliated with chronic kidney disease (CKD).
There is increasing evidence supporting the role of lipid or lipid-derived
mediators in the pathogenesis of CKD and other aging-related diseases.
To understand the role of lipids in various metabolic processes during
kidney aging, we conducted matrix-assisted laser desorption/ionization-imaging
mass spectrometry (MALDI-IMS) analysis in kidneys harvested from young
(2 months old, n = 3) and old mice (24 months old, n = 3). MALDI-IMS analysis showed an increase in ceramide
level and a decrease in sphingomyelin (SM) and phosphatidylcholine
(PC) levels in kidneys of old mice. The increased expression of cPLA2
and SMPD1 protein in aged kidney was confirmed by immunohistochemistry
and Western blot analysis. Our MALDI-IMS data showed the altered distribution
of lipids in aged kidney as indicative of aging-related functional
changes of the kidney. Combined analysis of MALDI-IMS and IHC confirmed
lipidomic changes and expression levels of responsible enzymes as
well as morphological changes.
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Noh, Sue Ah; Kim, Su-Mi; Park, Seon Hwa; Kim, Dong-Jin; Won Lee, Joon; Gyun Kim, Yang; et al. (2019). Alterations in
Lipid Profile of the Aging Kidney Identified
by MALDI Imaging Mass Spectrometry. ACS Publications. Collection. https://doi.org/10.1021/acs.jproteome.9b00108
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AUTHORS (10)
SN
Sue Ah Noh
SK
Su-Mi Kim
SP
Seon Hwa Park
DK
Dong-Jin Kim
JW
Joon Won Lee
YG
Yang Gyun Kim
JM
Ju-Young Moon
SL
Sung-Jig Lim
SL
Sang-Ho Lee
KK
Kwang Pyo Kim