Adaptive Hybrid Molecular Brushes Composed of Chitosan,
Polylactide, and Poly(N‑vinyl pyrrolidone)
for Support and Guiding Human Dermal Fibroblasts
Posted on 2020-06-25 - 20:14
Hybrid
molecular brushes (HMBs) are macromolecular constructs made
up of a backbone polymer and side-chain polymers with distinct properties.
They adapt to a changing microenvironment via the conformational mechanism
and thus may affect mammalian cell proliferation. Two biobenign HMBs
were synthesized in this work: (1) polylactide (PLA) grafted to the
chitosan (CHI) backbone to form chitosan-graft-polylactide
(CHI-g-PLA), a two-component molecular brush, and
(2) poly(N-vinyl pyrrolidone) (PNVP) grafted to chitosan
moieties of CHI-g-PLA to form a three-component HMB.
The molecular brushes were used to fabricate polymer coatings and
nanofibers, guiding the attachment and growth of human dermal fibroblasts
(HDFs) while silencing the response of macrophages to the scaffolds.
The exterior surface composition of the coatings can be switched by
exposure to solvents of different polarities: hydrophilic PNVP chains
upon exposure to water or hydrophobic PLA chains upon treatment by
anisole. Our experiments demonstrate substantial improvement of the
HDF cell attachment and proliferation on the surface of the HMBs as
compared to the parent polymers CHI, PLA, and PNVP. A Sirius Red assay
and immunofluorescence show that HMBs stimulate production of collagen
by HDF cells, which propagate on the polymer substrates revealing
well-developed focal adhesion structures. On the other hand, a low
attachment of macrophages is observed on the HMB surfaces, in particular
if HMBs are switched to the hydrophilic state, i.e., PNVP in the top
strata.
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Chawathe, Manasi; Asheghali, Darya; Minko, Sergiy; Jonnalagadda, Sriramakamal; Sidorenko, Alexander (2020). Adaptive Hybrid Molecular Brushes Composed of Chitosan,
Polylactide, and Poly(N‑vinyl pyrrolidone)
for Support and Guiding Human Dermal Fibroblasts. ACS Publications. Collection. https://doi.org/10.1021/acsabm.0c00217