la7b02887_si_001.pdf (473.75 kB)
pH Tunable and Divalent Metal Ion Tolerant Polymer Lipid Nanodiscs
journal contribution
posted on 2017-09-18, 00:00 authored by Thirupathi Ravula, Nathaniel Z. Hardin, Sudheer Kumar Ramadugu, Ayyalusamy RamamoorthyThe
development and applications of detergent-free membrane mimetics
have been the focus for the high-resolution structural and functional
studies on membrane proteins. The introduction of lipid nanodiscs
has attracted new attention toward the structural biology of membrane
proteins and also enabled biomedical applications. Lipid nanodiscs
provide a native lipid bilayer environment similar to the cell membrane
surrounded by a belt made up of proteins or peptides. Recent studies
have shown that the hydrolyzed form of styrene maleic anhydride copolymer
(SMA) has the ability to form lipid nanodiscs and has several advantages
over protein or peptide based nanodiscs. SMA polymer lipid nanodiscs
have become very important for structural biology and nanobiotechnological
applications. However, applications of the presently available polymer
nanodiscs are limited by their instability toward divalent metal ions
and acidic conditions. To overcome the limitations of SMA nanodiscs
and to broaden the potential applications of polymer nanodiscs, the
present study investigates the tunability of SMA polymer nanodiscs
by systematically modifying the maleic acid functional group. The
two newly developed polymers and subsequent lipid nanodiscs were characterized
using solid-state NMR, FT-IR, TEM, and DLS experiments. The pH dependence
and metal ion stability of these nanodiscs were studied using static
light scattering and FTIR. The reported polymer nanodiscs exhibit
unique pH dependent stability based on the modified functional group
and show a high tolerance toward divalent metal ions. We also show
these tunable nanodiscs can be used to encapsulate and stabilize a
polyphenolic natural product curcumin.
History
Usage metrics
Categories
Keywords
FT-IRmetal ion stabilitymembrane proteinsDLSDivalent Metal Ion Tolerant Polymer Lipid Nanodiscslipid nanodiscslipid bilayer environmentform lipid nanodiscsstyrene maleic anhydride copolymerFTIRTEMSMA polymer nanodiscsNMRSMA polymer lipid nanodiscspolymer nanodiscsdivalent metal ionsapplicationdetergent-free membrane mimeticspolymer nanodiscs exhibit
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC