pr9b00409_si_002.xlsx (329.99 kB)
pH Gradient Mitigation in the Leaf Cell Secretory Pathway Attenuates the Defense Response of Nicotiana benthamiana to Agroinfiltration
dataset
posted on 2019-12-12, 15:08 authored by Philippe
V. Jutras, Frank Sainsbury, Marie-Claire Goulet, Pierre-Olivier Lavoie, Rachel Tardif, Louis-Philippe Hamel, Marc-André D’Aoust, Dominique MichaudPartial neutralization of the Golgi lumen pH by the ectopic
expression
of influenza virus M2 proton channel is useful to stabilize acid-labile
recombinant proteins in plant cells, but the impact of pH gradient
mitigation on host cellular functions has not been investigated. Here,
we assessed the unintended effects of M2 expression on the leaf proteome
of Nicotiana benthamiana infiltrated
with the bacterial gene vector Agrobacterium tumefaciens. An isobaric tags for relative and absolute quantification quantitative
proteomics procedure was followed to compare the leaf proteomes of
plants agroinfiltrated with either an “empty” vector
or an M2-encoding vector. Leaves infiltrated with the empty vector
had a low soluble protein content compared to noninfiltrated control
leaves, associated with increased levels of stress-related proteins
but decreased levels of photosynthesis-associated proteins. M2 expression
partly compromised these effects of agroinfiltration to restore soluble
protein content in the leaf tissue, associated with restored levels
of photosynthesis-associated proteins and reduced levels of stress-related
proteins in the apoplast. These data illustrate the cell-wide influence
of the Golgi lumen pH homeostasis on the leaf proteome of N. benthamiana responding to microbial challenge.
They also underline the relevance of assessing the eventual unintended
effects of accessory proteins used to modulate specific cellular or
metabolic functions in plant protein biofactories.
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m 2 expressionpH Gradient Mitigationgene vector Agrobacterium tumefaciensLeaf Cell Secretory Pathway Attenuatesphotosynthesis-associated proteinsleaf proteomeinfluenza virus M 2 proton channelM 2 expressionM 2-encoding vectorAgroinfiltration Partial neutralizationpH gradient mitigationGolgi lumen pHNicotiana benthamianaplant protein biofactoriesprotein contentGolgi lumen pH homeostasis
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