posted on 2020-07-02, 17:12authored byChristina Liedert, Lauri Rannaste, Annukka Kokkonen, Olli-Heikki Huttunen, Ralph Liedert, Jussi Hiltunen, Leena Hakalahti
Lack
of functional integration and high manufacturing costs have
been identified as major challenges in commercialization of point-of-care
devices. In this study, roll-to-roll (R2R) fabrication process was
developed for large-scale manufacturing of disposable microfluidic
devices. The integrated, user-friendly device included a plasma separation
membrane for simple blood filtration, immobilized antibodies for specific
immunodetection, microfluidics for plasma transport and reagent mixing,
and a blister for actuation and waste storage. These functionalities
were designed to be compatible with R2R processing, which was demonstrated
using pilot-scale printing lines producing 60 devices in an hour.
The produced sensors enabled rapid (10 min) and sensitive (2 μg/mL)
fluorescence-based immunodetection of C-reactive protein from 20 μL
of whole blood.