American Chemical Society
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Roll-to-Roll Manufacturing of Integrated Immunodetection Sensors

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posted on 2020-07-02, 17:12 authored by Christina Liedert, Lauri Rannaste, Annukka Kokkonen, Olli-Heikki Huttunen, Ralph Liedert, Jussi Hiltunen, Leena Hakalahti
Lack of functional integration and high manufacturing costs have been identified as major challenges in commercialization of point-of-care devices. In this study, roll-to-roll (R2R) fabrication process was developed for large-scale manufacturing of disposable microfluidic devices. The integrated, user-friendly device included a plasma separation membrane for simple blood filtration, immobilized antibodies for specific immunodetection, microfluidics for plasma transport and reagent mixing, and a blister for actuation and waste storage. These functionalities were designed to be compatible with R2R processing, which was demonstrated using pilot-scale printing lines producing 60 devices in an hour. The produced sensors enabled rapid (10 min) and sensitive (2 μg/mL) fluorescence-based immunodetection of C-reactive protein from 20 μL of whole blood.