posted on 2024-08-15, 18:37authored byDaisuke Inoue
The
microtubule-kinesin biomolecular motor system, which is vital
for cellular function, holds significant promise for nanotechnological
applications. In vitro gliding assays have demonstrated
the ability to transport microcargo by propelling microtubules across
kinesin-coated surfaces. However, the uncontrolled directional motion
of microtubules has posed significant challenges, limiting the system’s
application for precise cargo delivery. Microfluidic devices provide
a means to direct microtubule movement through their geometric features.
Norland Optical Adhesive (NOA) is valued for its mold-free application
in microfluidic device fabrication; however, microtubules often climb
up channel walls, limiting controlled movement. In this study, a surface
passivation method for NOA is introduced, using polyethylene glycol
via a thiol–ene click reaction. This technique significantly
improved the directional control and concentration of microtubules
within NOA microchannels. This approach presents new possibilities
for the precise application of biomolecular motors in nanotechnology,
enabling advancements in the design of microfluidic systems for complex
biomolecular manipulations.