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Superparamagnetic Iron Oxide Nanoparticles with Large Magnetic Saturation and High Particle Photon Counts for Super-Resolution Imaging of Lysosomes

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posted on 2022-03-04, 22:33 authored by Aditya Yadav, Chethana Rao, Kush Kaushik, Farhan Anjum, Shagun Sharma, Chayan Kanti Nandi
Superparamagnetic iron oxide nanoparticles (SPIONs) have become benchmark materials for their large magnetic saturation and excellent T2 contrast. Despite the large magnetic saturation that arises due to the enormous iron content, incorporating a fluorescent nature for their use as multimodal probes drastically reduces the magnetic saturation. To achieve the desired magnetic saturation in fluorescence-decorated SPIONs, the size is increased substantially to hundreds of nanometers. Here, we rationally design fluorescent SPIONs of size ∼16 nm with significant magnetic saturation and high single-particle photon counts used for optical-based super-resolution microscopy. The SPIONs were highly specific to stain and image the lysosomes of HeLa cells with high contrast and were capable of providing super-resolution radial fluctuation (SRRF) imaging of lysosomes down to 130 nm, which is much lower than the diffraction limit in light microscopy.

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