jz6b02098_si_003.avi (2.5 MB)
Super Temporal-Resolved Microscopy (STReM)
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posted on 2016-10-24, 00:00 authored by Wenxiao Wang, Hao Shen, Bo Shuang, Benjamin
S. Hoener, Lawrence J. Tauzin, Nicholas A. Moringo, Kevin F. Kelly, Christy F. LandesSuper-resolution
microscopy typically achieves high spatial resolution,
but the temporal resolution remains low. We report super temporal-resolved
microscopy (STReM) to improve the temporal resolution of 2D super-resolution
microscopy by a factor of 20 compared to that of the traditional camera-limited
frame rate. This is achieved by rotating a phase mask in the Fourier
plane during data acquisition and then recovering the temporal information
by fitting the point spread function (PSF) orientations. The feasibility
of this technique is verified with both simulated and experimental
2D adsorption/desorption and 2D emitter transport. When STReM is applied
to measure protein adsorption at a glass surface, previously unseen
dynamics are revealed.