ph5b00647_si_002.avi (5.19 MB)
Super-Resolution Chemical Imaging with Plasmonic Substrates
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posted on 2016-02-17, 00:00 authored by Aeli P. Olson, Christopher
T. Ertsgaard, Sarah N. Elliott, Nathan C. LindquistWe demonstrate super-resolution chemical
imaging with plasmonic
nanoholes via surface-enhanced Raman spectroscopy (SERS). Due to large
field enhancements, blinking behavior of SERS hot spots was observed
and processed using a stochastic optical reconstruction microscopy
(STORM) algorithm. This enabled localization to within 10 nm and high-resolution
imaging. However, illumination of the sample with a static laser beam
produced only SERS hot spots in fixed locations, leaving noticeable
gaps in the final images. By randomly altering the phase profile of
the incident beam with a simple optical diffuser, the hot spots were
shifted across the plasmonic surface to illuminate different areas
of the sample, thereby rendering a final image without the gaps. A
tunable band-pass filter was used to preserve spectral information,
allowing chemical contrast imaging. Images were then compared to those
obtained with a scanning electron microscope. Finally, we show that
super-resolution SERS images can also be obtained with our dynamic
illumination technique on even the most basic plasmonic substrate:
as-deposited rough silver films. These results show significant potential
for the use of simple plasmonic substrates with straightforward illumination
and collection schemes for super-resolution chemical imaging.
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plasmonic substratesimageplasmonic substrateplasmonic nanoholesSERSsamplefield enhancementslaser beamincident beamPlasmonic Substrates10 nmgapphase profilescanning electron microscopecollection schemesreconstruction microscopysilver filmsresults showillumination techniqueplasmonic surfacechemical contrast imaging
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