Specific Detection of Integrin αvβ3 by Light-Up Bioprobe with Aggregation-Induced Emission Characteristics
mediaposted on 13.06.2012, 00:00 by Haibin Shi, Jianzhao Liu, Junlong Geng, Ben Zhong Tang, Bin Liu
Specific bioprobes with fluorescence turn-on response are highly desirable for high contrast biosensing and imaging. In this work, we developed a new generation bioprobe by integrating tetraphenylsilole, a fluorogenic unit with aggregation-induced emission (AIE) characteristic, with cyclic arginine–glycine–aspartic acid tripeptide (cRGD), a targeting ligand to integrin αvβ3 receptor. Emission of the AIE probe is switched on upon its specific binding to integrin αvβ3, which allows quantitative detection of integrin αvβ3 in solution and real-time imaging of the binding process between cRGD and integrin αvβ3 on cell membrane. The probe can be used for tracking integrin αvβ3 and for identifying integrin αvβ3-positive cancer cells.