Single-Shot Quantitative Polarization Imaging of Complex Birefringent Structure Dynamics
mediaposted on 2021-11-17, 23:04 authored by Baoliang Ge, Qing Zhang, Rui Zhang, Jing-Tang Lin, Po-Hang Tseng, Che-Wei Chang, Chen-Yuan Dong, Renjie Zhou, Zahid Yaqoob, Irmgard Bischofberger, Peter T. C. So
Polarization light microscopes are powerful tools for probing molecular order and orientation in birefringent materials. While a number of polarization microscopy techniques are available to access steady-state properties of birefringent samples, quantitative measurements of the molecular orientation dynamics on the millisecond time scale have remained a challenge. We propose polarized shearing interference microscopy (PSIM), a single-shot quantitative polarization imaging method, for extracting the retardance and orientation angle of the laser beam transmitting through optically anisotropic specimens with complex structures. The measurement accuracy and imaging performance of PSIM are validated by imaging a birefringent resolution target and a bovine tendon specimen. We demonstrate that PSIM can quantify the dynamics of a flowing lyotropic chromonic liquid crystal in a microfluidic channel at an imaging speed of 506 frames per second (only limited by the camera frame rate), with a field-of-view of up to 350 × 350 μm2 and a diffraction-limit spatial resolution of ∼2 μm. We envision that PSIM will find a broad range of applications in quantitative material characterization under dynamical conditions.
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∼ 2 μmprobing molecular orderpolarization microscopy techniquesoptically anisotropic specimensmillisecond time scalelimit spatial resolutionlaser beam transmittingbovine tendon specimenquantitative material characterizationbirefringent resolution target2 </ supmolecular orientation dynamicsquantitative measurementsbirefringent samplesbirefringent materialsstate propertiespowerful toolsorientation anglemicrofluidic channelmeasurement accuracydynamical conditionscomplex structuresbroad rangeaccess steady