American Chemical Society
bc400291f_si_002.avi (41.45 MB)

Rational Design of Chemical Ligands for Selective Mitochondrial Targeting

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posted on 2013-09-18, 00:00 authored by Silvie Rimpelová, Tomáš Bříza, Jarmila Králová, Kamil Záruba, Zdeněk Kejík, Ivana Císařová, Pavel Martásek, Tomáš Ruml, Vladimír Král
The rational design of molecules with selective intracellular targeting is a great challenge for contemporary chemistry and life sciences. Here, we demonstrate a rational approach to development of compartment-specific fluorescent dyes from the γ-aryl substituted pentamethine family. These novel dyes exhibit an extraordinary affinity and selectivity for cardiolipin in inner mitochondrial membrane and possess excellent photostability, fluorescent properties, and low phototoxicity. Selective imaging of live and fixed mitochondria was achieved in various cell lines using nanomolar concentrations of these dyes. Their high localization specificity and low toxicity enables study of morphological changes, structural complexity, and dynamics of mitochondria playing a pivotal role in many pathological diseases. These far-red emitting dyes could also serve in a variety of biomedical applications.