am8b20315_si_002.avi (3.45 MB)
Nanomechanical Microfluidic Mixing and Rapid Labeling of Silica Nanoparticles using Allenamide-Thiol Covalent Linkage for Bioimaging
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posted on 2019-01-09, 00:00 authored by Sivaramapanicker Sreejith, Rahul Kishor, Ata Abbas, Rijil Thomas, Trifanny Yeo, Vivek Damodar Ranjan, Ramanathan Vaidyanathan, Yen Peng Seah, Bengang Xing, Zhenfeng Wang, Li Zeng, Yuanjin Zheng, Chwee Teck LimRapid surface functionalization
of nanomaterials using covalent linkage following “green chemistry”
remains challenging, and the quest for developing simple protocols
is persisting. We report a nanomechanical microfluidic approach for
the coupling of allenamide functionalized organic derivatives on the
surface of thiol-modified silica nanoparticles using allenamide-thiol
chemistry. The coupling principle involves the use of a microfluidic
surface acoustic wave device that generates acoustic streaming-based
chaotic fluid micromixing that enables mixing of laterally flowing
fluids containing active components. This approach was used to demonstrate
the direct surface labeling of thiol-modified silica nanoparticles
using a selected group of modified fluorescent tags containing allenamide
handles and achieved a total labeling efficiency of 83–90%.
This green approach enabled a highly efficient surface functionalization
under aqueous conditions, with tunable control over the conjugation
process via the applied field. The dye-labeled silica particles were
characterized using various analytical techniques and found to be
biocompatible with potential in live cell bioimaging. It is envisaged
that this bioconjugation strategy will find numerous applications
in the field of bioimaging and drug delivery.
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bioconjugation strategywave devicesurface functionalizationSilica Nanoparticlesmicrofluidic surfacecell bioimagingAllenamide-Thiol Covalent LinkageNanomechanical Microfluidic Mixingfluid micromixingcovalent linkageBioimaging Rapid surface functionalizationdrug deliveryconjugation processtunable controldye-labeled silica particlesthiol-modified silica nanoparticlesallenamide functionalizedallenamide-thiol chemistrynanomechanical microfluidic approach
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