ac6b04139_si_003.mpg (6.11 MB)
Download fileNanocomputed Tomography Imaging of Bacterial Alkaline Phosphatase Activity with an Iodinated Hydrogelator
media
posted on 2016-12-07, 00:00 authored by Zhen Zheng, Anming Tang, Yong Guan, Liang Chen, Fuqiang Wang, Peiyao Chen, Weijuan Wang, Yufeng Luo, Yangchao Tian, Gaolin LiangAlkaline phosphatase
(ALP) is an important enzyme, but direct imaging
of ALP activity with high spatiotemporal resolution remains challenging.
In this work, we rationally designed an iodinated hydrogelator precursor
Nap-Phe-Phe(I)-Tyr(H2PO3)-OH (1P) which self-assembles into nanofibers to form hydrogel under the
catalysis of ALP. With this property of concentrating iodine atoms
at the locations of ALP, 1P was successfully applied
for direct nanocomputed tomography (nano-CT) imaging of ALP activity
in bacteria for the first time. We envision that, on the basis of
this pioneering work, new hydrogelators containing more iodine atoms
(e.g., five iodine atoms in 1P) will be designed for
better nano-CT imaging of ALP activity with higher CT contrast in
the near future.