American Chemical Society
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Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters

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posted on 2022-01-13, 13:05 authored by Vincent Grenier, Kayli N. Martinez, Brittany R. Benlian, Derek M. García-Almedina, Benjamin K. Raliski, Steven C. Boggess, Johnathan C. Maza, Samantha J. Yang, Anneliese M. M. Gest, Evan W. Miller
Voltage-sensitive fluorescent reporters can reveal fast changes in the membrane potential in neurons and cardiomyocytes. However, in many cases, illumination in the presence of the fluorescent reporters results in disruptions to the action potential shape that limits the length of recording sessions. We show here that a molecular prosthetic approach, previously limited to fluorophores, rather than indicators, can be used to substantially prolong imaging in neurons and cardiomyocytes.