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Download fileMolecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters
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posted on 2022-01-13, 13:05 authored by Vincent Grenier, Kayli N. Martinez, Brittany R. Benlian, Derek M. García-Almedina, Benjamin K. Raliski, Steven C. Boggess, Johnathan C. Maza, Samantha J. Yang, Anneliese M. M. Gest, Evan W. MillerVoltage-sensitive
fluorescent reporters can reveal fast changes
in the membrane potential in neurons and cardiomyocytes. However,
in many cases, illumination in the presence of the fluorescent reporters
results in disruptions to the
action potential shape that limits the length of recording sessions.
We show here that a molecular prosthetic approach, previously limited
to fluorophores, rather than indicators, can be used to substantially
prolong imaging in neurons and cardiomyocytes.
