ac1c00828_si_002.mp4 (2.67 MB)
Label-Free Probing of Molecule Binding Kinetics Using Single-Particle Interferometric Imaging
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posted on 2021-05-24, 20:13 authored by Di Jiang, Xiaona Zhao, Yi-Nan Liu, Hai-Bo Chen, Wen-Li Lv, Chen Qian, Xian-Wei LiuProbing
molecular interactions is critical for screening drugs,
detecting pollutants, and understanding biological processes at the
molecular level, but these interactions are difficult to detect, especially
for small molecules. A label-free optical imaging technology that
can detect molecule binding kinetics is presented, in which free-moving
particles are driven into oscillations with an alternating electrical
field and the interferometric scattering patterns of the particles
are imaged via an optical imaging method. By tracking the charge-sensitive
variations in the oscillation amplitude with sub-nanometer precision,
the small molecules and metal ions binding to the surface as well
as protein–protein binding kinetics were measured. The capability
of the label-free measurement of molecular interactions can provide
a promising platform for screening small-molecule drugs, probing conformational
changes in proteins, and detecting environmental pollutants.
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charge-sensitive variationsSingle-Particle Interferometric Imagingmetal ions bindingfree-moving particlesproteinscreening drugsMolecule Binding Kineticsoscillation amplitudemolecule binding kineticsimaging methodpollutantinteractionsub-nanometer precisionimaging technologyscreening small-molecule drugslabel-free measurement