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Individual Coating of Entomopathogenic Nematodes with Titania (TiO2) Nanoparticles Based on Oil-in-Water Pickering Emulsion: A New Formulation for Biopesticides
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posted on 2022-10-13, 09:31 authored by Liliya Kotliarevski, Raz Cohen, Jayashree Ramakrishnan, Shaohui Wu, Karthik Ananth Mani, Reut Amar-Feldbaum, Noga Yaakov, Einat Zelinger, Eduard Belausov, David Shapiro-Ilan, Itamar Glazer, Dana Ment, Guy MechrezThis
study presents a new eco-friendly formulation of
entomopathogenic
nematodes (EPNs) based on individual coating of EPNs with titanium
dioxide (TiO2) nanoparticles (NPs) and mineral oil via
oil-in-water Pickering emulsions. Mineral oil-in-water emulsions stabilized
by amine-functionalized titanium dioxide (TiO2-NH2) particles were prepared. 40:60 and 50:50 oil–water volume
ratios using 2 wt % TiO2-NH2 particles were
found to be the most stable emulsions with a droplet size suitable
for the formulation and were further studied for their toxicity against
the incorporated EPNs. Carboxyfluorescein was covalently bonded to
TiO2-NH2 NPs, and the resulting composite was
observed via fluorescence confocal microscopy. The dry coating was
evaluated using SEM and confocal microscopy, which showed significant
nematode coverage by the particles and oil. The final formulation
was biocompatible with the studied EPNs, where the viability of the
EPNs in the formulation was equivalent to control aqueous suspension
after 120 days. Finally, yields of nematodes from infected Galleria mellonella cadavers collected for 150 days
showed no significant differences (P > 0.05) using
the tested emulsions compared to the control containing nematodes
in water.
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significant differences (<droplet size suitablecontrol aqueous suspensiontested emulsions comparedwater pickering emulsion150 days showedwater pickering emulsionswater emulsions stabilizedfunctionalized titanium dioxideevaluated using semcontrol containing nematodes2 subtitanium dioxidestable emulsionsp 120 daysstudy presentsresulting compositenew ecoindividual coatingentomopathogenic nematodesdry coatingcovalently bondedconfocal microscopy
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