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Download fileIn-Channel Printing-Device Opening Assay for Micropatterning Multiple Cells and Gene Analysis
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posted on 2015-02-17, 00:00 authored by Hao Zhou, Liang Zhao, Xueji ZhangHerein we report an easy but versatile
method for patterning different
cells on a single substrate by using a microfluidic approach that
allows not only spatial and temporal control of multiple microenvironments
but also retrieval of specific treated cells to profile their expressed
genetic information at around 10-cell resolution. By taking advantages
of increased surface area of gold nanoparticles on a poly(dimethylsiloxane)
(PDMS) coated substrate, cell adhesive-promotive protein, human fibronectin
(hFN) can be significantly accumulated on designed regions where cells
can recognize the protein and spread out. Moreover, the whole device
can be easily opened by hand without any loss of patterned cells which
could be retrieved by mouth-pipet. Consequently, we demonstrate the
possibility of analyzing the difference of gene expression patterns
between wild type MCF-7 cell and MCF/Adr (drug-resistant cell line)
from less than 400 cells in total for a single comprehensive assay,
including parallel experiments, controls, and multiple dose treatments.
Certain genes, especially the P-glycoprotein coding gene (ABCB1),
show high expression level in resistant cells compared with the wild
type, suggesting a possible pathway that may contribute to the antidrug
mechanism.