cm3c00587_si_002.avi (33 MB)
Ficin–Cyclodextrin-Based Docking Nanoarchitectonics of Self-Propelled Nanomotors for Bacterial Biofilm Eradication
Version 2 2023-05-18, 12:03
Version 1 2023-05-09, 18:03
mediaposted on 2023-05-18, 12:03 authored by Miglė Žiemytė, Andrea Escudero, Paula Díez, María D. Ferrer, Jose R. Murguía, Vicente Martí-Centelles, Alex Mira, Ramón Martínez-Máñez
Development of bioinspired nanomotors showing effective propulsion and cargo delivery capabilities has attracted much attention in the last few years due to their potential use in biomedical applications. However, implementation of this technology in realistic settings is still a barely explored field. Herein, we report the design and application of a multifunctional gated Janus platinum–mesoporous silica nanomotor constituted of a propelling element (platinum nanodendrites) and a drug-loaded nanocontainer (mesoporous silica nanoparticle) capped with ficin enzyme modified with β-cyclodextrins (β-CD). The engineered nanomotor is designed to effectively disrupt bacterial biofilms via H2O2-induced self-propelled motion, ficin hydrolysis of the extracellular polymeric matrix (EPS) of the biofilm, and controlled pH-triggered cargo (vancomycin) delivery. The effective synergic antimicrobial activity of the nanomotor is demonstrated in the elimination of Staphylococcus aureus biofilms. The nanomotor achieves 82% of EPS biomass disruption and a 96% reduction in cell viability, which contrasts with a remarkably lower reduction in biofilm elimination when the components of the nanomotors are used separately at the same concentrations. Such a large reduction in biofilm biomass in S. aureus has never been achieved previously by any conventional therapy. The strategy proposed suggests that engineered nanomotors have great potential for the elimination of biofilms.
strategy proposed suggestsmesoporous silica nanoparticleextracellular polymeric matrixbased docking nanoarchitectonicsbarely explored fieldattracted much attentionficin – cyclodextrinficin enzyme modifiedremarkably lower reductionnanomotor achieves 822 subeps biomass disruptioncargo delivery capabilitiesstaphylococcus aureus biofilmsficin hydrolysistriggered cargobiofilm biomassyears dueused separatelyrealistic settingspropelling elementpropelled motionpotential useplatinum nanodendritesloaded nanocontainerlarge reductiongreat potentialengineered nanomotorconventional therapycontrolled phcell viabilitycd ).biomedical applicationsachieved previously