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Evaluation of the Capitainer‑B Microfluidic Device as a New Hematocrit-Independent Alternative for Dried Blood Spot Collection
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posted on 2018-11-16, 18:34 authored by Sofie Velghe, Christophe P. StoveThe
hematocrit-bias still remains one of the most discussed issues
when it comes to dried blood spot (DBS) analysis. Therefore, many
attempts to cope with this issue have been made, among which the development
of novel sampling tools such as the Capitainer-B (further referred
to as MF (microfluidic)-DBS) devices. These are designed to allow
a straightforward absorption of a fixed volume (13.5 μL) of
blood by a preperforated paper disc, which can be analyzed afterward.
The aim of this study was to evaluate the potential of these devices
to nullify the hematocrit-based area bias and to investigate whether
the amount of blood applied has an influence on the device performance.
An LC-MS/MS method for the quantification of caffeine and paraxanthine
in MF-DBS was fully validated, meeting all preset acceptance criteria.
In a next step, using a set of 133 authentic, venous patient samples
with a hematocrit range of 18.8–55.0, concentrations of both
compounds in MF-DBS were compared to those in corresponding partial-punch
pipetted DBS (PI-DBS) and liquid blood samples. When compared to blood
as a reference, the concentrations obtained in MF-DBS were not affected
by a bias in function of the evaluated hematocrit, in contrast to
those obtained from partial-punch PI-DBS. Furthermore, analysis of
samples resulting from spiking different volumes of whole blood at
different hematocrit levels, revealed that the amount of blood applied
at the device inlet has no influence on the performance of the devices.
Therefore, it can be concluded from this study, being the first in
which the impact of the hematocrit and the applied volume is evaluated
by analyzing authentic, venous patient samples, that MF-DBS devices
effectively assist in eliminating the hematocrit-based area bias,
independently from the applied blood volume.
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acceptance criteriablood volumehematocrit levelsvenous patient samplesMF-DBS devicesdevice performancedevice inletpartial-punch pipetted DBSblood samplespartial-punch PI-DBSpreperforated paper discNew Hematocrit-IndependentLC-MShematocrit-based area biasBlood Spot Collectionnovel sampling toolsblood spothematocrit range
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