posted on 2021-12-23, 19:34authored byChao Zhou, Shaoyang Zhang, Jing Ai, Ping Li, Yanjie Zhao, Baojie Li, Lu Han, Yingying Duan, Shunai Che
The
enantioselective interactions between cells and chiral materials
would be a crucial factor in homochiral organisms. Herein, we report
that pure inorganic chiral mesostructured hydroxyapatite films (CMHAPFs)
exhibit enantioselectivity in the regulation of cell behaviors. CMHAPFs
were fabricated with tartaric acid (TTA) as a symmetry-breaking agent,
and five levels of fractal-like chirality existed: primary twisted
“nanoneedles” with atomically distorted crystal lattices,
secondary “nanoflakes” with a helical arrangement of
“nanoneedles”, tertiary “nanoplates” with
a helical arrangement of “nanoflakes”, quaternary “nanopetals”
with a helical arrangement of “nanoplates”, and quinary
“flowers” consisting of helically arranged “nanopetals”.
The CMHAPFs synthesized with L-TTA were advantageous in cell adhesion,
cell proliferation, and osteogenic differentiation of primary mesenchymal
stem cells, whereas the opposite effect was observed in the CMHAPFs
synthesized with D-TTA. This enantioselectivity was speculated to
be due to the stereo matching between the hierarchical chiral cell
surfaces and multilevel chiral mesostructures of CMHAPFs.