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Download fileDimensionality of Rolled-up Nanomembranes Controls Neural Stem Cell Migration Mechanism
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posted on 2015-12-17, 09:05 authored by Britta Koch, Anne K. Meyer, Linda Helbig, Stefan M. Harazim, Alexander Storch, Samuel Sanchez, Oliver G. SchmidtWe
employ glass microtube structures fabricated by rolled-up nanotechnology
to infer the influence of scaffold dimensionality and cell confinement
on neural stem cell (NSC) migration. Thereby, we observe a pronounced
morphology change that marks a reversible mesenchymal to amoeboid
migration mode transition. Space restrictions preset by the diameter
of nanomembrane topography modify the cell shape toward characteristics
found in living tissue. We demonstrate the importance of substrate
dimensionality for the migration mode of NSCs and thereby define rolled-up
nanomembranes as the ultimate tool for single-cell migration studies.