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Download fileDietary Fe3O4 Nanozymes Prevent the Injury of Neurons and Blood–Brain Barrier Integrity from Cerebral Ischemic Stroke
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posted on 2020-12-21, 16:06 authored by Bing Chun Yan, Jianwen Cao, Jiajia Liu, Yunhao Gu, Zhuobin Xu, Dandan Li, Lizeng GaoCerebral
ischemic stroke stimulates excessive reactive oxygen species,
which lead to blood–brain-barrier disruption, neuron death,
and aggravated cerebral infarction. Thus, it is critical to develop
an antioxidant strategy for stroke treatment. Herein, we report a
dietary strategy to promote stroke healing using iron oxide (Fe3O4) nanoparticles with intrinsic enzyme-like activities.
We find that Fe3O4 nanozymes exhibit triple
enzyme-like activities, peroxidase, catalase, and superoxide dismutase,
thus potentially possessing the ability to regulate the ROS level.
Importantly, intragastric administration of PEG-modified Fe3O4 nanozymes significantly reduces cerebral infarction
and neuronal death in a rodent model following cerebral ischemic stroke. Ex vivo analysis shows that PEG-modified Fe3O4 nanozymes localize in the cerebral vasculature, ameliorate
local redox state with decreased malondialdehyde and increased Cu/Zn
SOD, and facilitate blood–brain-barrier recovery by elevating
ZO-1 and Claudin-5 in the hippocampus. Altogether, our results suggest
that dietary PEG-modified Fe3O4 nanozymes can
facilitate blood–brain-barrier reconstruction and protect neurons
following ischemic stroke.