Detection of Enzyme Inhibitors in Crude Natural Extracts Using Droplet-Based Microfluidics Coupled to HPLC
mediaposted on 04.04.2017, 00:00 by Abraham Ochoa, Enrique Álvarez-Bohórquez, Eduardo Castillero, Luis F. Olguin
Natural product screening for new bioactive compounds can greatly benefit from low reagents consumption and high throughput capacity of droplet-based microfluidic systems. However, the creation of large droplet libraries in which each droplet carries a different compound is a challenging task. A possible solution is to use an HPLC coupled to a droplet generating microfluidic device to sequentially encapsulate the eluting compounds. In this work we demonstrate the feasibility of carrying out enzyme inhibiting assays inside nanoliter droplets with the different components of a natural crude extract after being separated by a coupled HPLC column. In the droplet formation zone, the eluted components are mixed with an enzyme and a fluorogenic substrate that permits to follow the enzymatic reaction in the presence of each chromatographic peak and identify those inhibiting the enzyme activity. Using a fractal shape channel design and automated image analysis, we were able to identify inhibitors of Clostridium perfringens neuraminidase present in a root extract of the Pelargonium sidoides plant. This work demonstrates the feasibility of bioprofiling a natural crude extract after being separated in HPLC using microfluidic droplets online and represents an advance in the miniaturization of natural products screening.
Read the peer-reviewed publication
products screeningCrude Natural Extractseluting compoundsPelargonium sidoides plantimage analysisEnzyme Inhibitorsfluorogenic substratedroplet formation zonenanoliter dropletsfractal shape channel designdroplet-based microfluidic systemsbioactive compoundsHPLC Natural product screeningcomponentClostridium perfringens neuraminidaseenzyme activityHPLC columnDroplet-Based Microfluidicsmicrofluidic devicefeasibilitymicrofluidic dropletsthroughput capacitydroplet librariescrudereagents consumption