Chemical Modulation of in Vivo Macrophage Function with Subpopulation-Specific Fluorescent Prodrug Conjugates
mediaposted on 2017-08-30, 18:21 authored by Antonio Fernandez, Matthieu Vermeren, Duncan Humphries, Ramon Subiros-Funosas, Nicole Barth, Lara Campana, Alison MacKinnon, Yi Feng, Marc Vendrell
Immunomodulatory agents represent one of the most promising strategies for enhancing tissue regeneration without the side effects of traditional drug-based therapies. Tissue repair depends largely on macrophages, making them ideal targets for proregenerative therapies. However, given the multiple roles of macrophages in tissue homeostasis, small molecule drugs must be only active in very specific subpopulations. In this work, we have developed the first prodrug–fluorophore conjugates able to discriminate closely related subpopulations of macrophages (i.e., proinflammatory M1 vs anti-inflammatory M2 macrophages), and employed them to deplete M1 macrophages in vivo without affecting other cell populations. Selective intracellular activation and drug release enabled simultaneous fluorescence cell tracking and ablation of M1 macrophages in vivo, with the concomitant rescue of a proregenerative phenotype. Ex vivo assays in human monocyte-derived macrophages validate the translational potential of this novel platform to develop chemical immunomodulatory agents as targeted therapies for immune-related diseases.
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cell populationsimmune-related diseasesTissue repairtissue regenerationSelective intracellular activationM 1 macrophagesproregenerative phenotypefluorescence cellproregenerative therapiesmolecule drugsVivo Macrophage Functiondrug-based therapiesdrug releasemonocyte-derived macrophagesnovel platformtherapychemical immunomodulatory agentschemical ModulationEx vivo assaystissue homeostasisProdrug Conjugates Immunomodulatory agentsside effects