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Download fileChemical Constituents of Mangifera indica and Their Antiausterity Activity against the PANC‑1 Human Pancreatic Cancer Cell Line
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posted on 2016-07-28, 18:50 authored by Hai Xuan Nguyen, Truong
Nhat Van Do, Tho Huu Le, Mai Thanh Thi Nguyen, Nhan Trung Nguyen, Hiroyasu Esumi, Suresh AwaleHuman pancreatic cancer cell lines
such as PANC-1 have an altered
metabolism, enabiling them to tolerate and survive under extreme conditions
of nutrient starvation. The search for candidates that inhibit their
viability during nutrition starvation represents a novel antiausterity
strategy in anticancer drug discovery. A methanol extract of the bark
of Mangifera indica was found to inhibit the survival
of PANC-1 human pancreatic cancer cells preferentially under nutrient-deprived
conditions with a PC50 value of 15.5 μg/mL, without
apparent toxicity, in normal nutrient-rich conditions. Chemical investigation
on this bioactive extract led to the isolation of 19 compounds (1–19), including two new cycloartane-type
triterpenes, mangiferolate A (1) and mangiferolate B
(2). The structures of 1 and 2 were determined by NMR spectroscopic analysis. Among the isolated
compounds, mangiferolate B (2) and isoambolic acid (12) exhibited potent preferential cytotoxicity against PANC-1
human pancreatic cancer cells under the nutrition-deprived condition
with PC50 values of 11.0 and 4.8 μM, respectively.