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Characterization of the Hydration Process of Phospholipid-Mimetic Polymers Using Air-Injection-Mediated Liquid Exclusion Methods

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posted on 12.05.2020, 14:08 by Risa Katayama, Nobuyuki Tanaka, Yusuke Takagi, Kohei Shiraishi, Yo Tanaka, Akikazu Matsumoto, Chie Kojima
2-Methacryloyloxyethyl phosphorylcholine (MPC) polymers including hydrophobic units such as poly­(MPC-co-butyl methacrylate) (PMB) and poly­(MPC-co-dodecyl methacrylate) (PMD) are used as coating agents for medical devices because of their antifouling effects. In this study, the whole hydration process of MPC polymer-coated surfaces was investigated using air-injection-mediated liquid exclusion (AILE) methods in which the liquid exclusion diameter during air injection was correlated to the water-repelling property. The prejetted and standard AILE methods showed the initial change from a dry to a wet state and the swelling behaviors of the MPC polymers, respectively. The liquid exclusion diameter of the MPC polymer-coated surfaces increased with an increase in the immersion time in various aqueous solutions such as deionized water, phosphate-buffered saline (PBS), and cell culture media. Moreover, the liquid exclusion diameter of the PMD-coated surface was larger than that of the PMB-coated one. Ellipsometry directly indicated the polymer layers swollen in water. Scanning probe microscopy (SPM) revealed that nanosized protuberances were formed in water, especially at the PMD-coated surface. The different swelling behaviors of these MPC polymer-coated surfaces affected the liquid exclusion diameters. Thus, the AILE methods are a powerful tool to elucidate the hydration process in various liquid media.