posted on 2024-02-12, 07:30authored byChenchi Gong, Jun Wang, Faqiang Tang, Dongmei Tong, Ziyi Wang, Zijie Zhou, Renjie Ruan, Jin Zhang, Jibin Song, Huanghao Yang
Large osseous void, postsurgical neoplastic recurrence,
and slow
bone-cartilage repair rate raise an imperative need to develop functional
scaffold in clinical osteosarcoma treatment. Herein, a bionic bilayer
scaffold constituting croconaine dye-polyethylene glycol@sodium alginate
hydrogel and poly(l-lactide)/hydroxyapatite polymer matrix
is fabricated to simultaneously achieve a highly efficient killing
of osteosarcoma and an accelerated osteochondral regeneration. First,
biomimetic osteochondral structure along with adequate interfacial
interaction of the bilayer scaffold provide a structural reinforcement
for transverse osseointegration and osteochondral regeneration, as
evidenced by upregulated specific expressions of collagen type-I,
osteopontin, and runt-related transcription factor 2. Meanwhile, thermal
ablation of the synthesized nanoparticles and mitochondrial dysfunction
caused by continuously released hydroxyapatite induce residual tumor
necrosis synergistically. To validate the capabilities of inhibiting
tumor growth and promoting osteochondral regeneration of our proposed
scaffold, a novel orthotopic osteosarcoma model simulating clinical
treatment scenarios of bone tumors is established on rats. Based on
amounts of in vitro and in vivo results,
an effective killing of osteosarcoma and a suitable osteal-microenvironment
modulation of such bionic bilayer composite scaffold are achieved,
which provides insightful implications for photonic hyperthermia therapy
against osteosarcoma and following osseous tissue regeneration.