nl060598u_si_001.avi (49.18 MB)

Bioengineered Flagella Protein Nanotubes with Cysteine Loops:  Self-Assembly and Manipulation in an Optical Trap

Download (49.18 MB)
posted on 13.09.2006 by Mudalige Thilak Kumara, Narayanan Srividya, Subra Muralidharan, Brian C. Tripp
An E. coli flagellin protein, termed FliTrx, was investigated for use as a novel form of self-assembling protein nanotube. This protein was genetically engineered to display constrained peptide loops with a series of different thiol, cationic, anionic, and imidazole functional groups. “Cys-loop” thiol variants consisting of 6 and 12 cysteine residues were isolated in the form of disulfide-linked nanotube bundles, a novel nanomaterial. Bundles were characterized by fluorescence microscopy, transmission electron microscopy, and optical trapping.