nn6b01415_si_003.avi (1004.07 kB)
Acoustically Propelled Nanomotors for Intracellular siRNA Delivery
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posted on 2016-03-29, 00:00 authored by Berta Esteban-Fernández de Ávila, Chava Angell, Fernando Soto, Miguel Angel Lopez-Ramirez, Daniela F. Báez, Sibai Xie, Joseph Wang, Yi ChenAn effective intracellular gene silencing
strategy based on acoustically
propelled nanowires modified with an interfering RNA’s (siRNA)
payload is described. The gold nanowires (AuNW) are wrapped with a
Rolling Circle Amplification (RCA) DNA strand, which serves to anchor
the siRNA therapy. The ultrasound (US)-powered propulsion of the AuNW
leads to fast internalization and rapid intracellular movement and
hence to an accelerated siRNA delivery and silencing response. To
optimize the micromotor gene silencing procedure, the influence of
motion, time, and siRNA dosage was investigated, leading up to a 94%
silencing after few minutes treatment with US-propelled siRNA–AuNWs,
and to a dramatic (∼13-fold) improvement in the silencing response
compared to the static modified nanowires. The ability of the nanomotor-based
method for gene silencing has been demonstrated by measuring the GFP
silencing response in two different cell lines (HEK-293 and MCF-7)
and using detailed control experiments. The viability of the cells
after the nanomotors treatment was examined using the MCF-7 cancer
cell line. The use of DNA structures carried by the US-propelled nanomotors
for gene silencing represents an efficient tool that addresses the
challenges associated with RNA transportation and intracellular delivery.
Future implementation of nanomachines in gene therapy applications
can be expanded into a co-delivery platform for therapeutics.
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DNA structuresfuture implementationsiRNA therapysiRNA dosageintracellular deliverycell linescontrol experimentsDNA strandRolling Circle AmplificationsiRNA deliveryAcoustically Propelled NanomotorsHEKnanomotors treatmentmicromotor geneminutes treatmentgold nanowiresRCAIntracellular siRNA Deliveryintracellular movementresponsegene therapy applicationsMCFGFPintracellular geneRNA transportation
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