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A Novel Synthetic Peptide Polymer with Cyclic RGD Motifs Supports Serum-Free Attachment of Anchorage-Dependent Cells

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posted on 17.09.2008, 00:00 by K. Markó, M. Ligeti, G. Mezo″, N. Mihala, E. Kutnyánszky, É. Kiss, F. Hudecz, E. Madarász
Cell adhesivity is a basic biological principle, which provides mechanisms for construction of multicellular organisms, tissue genesis, migration and individual cell survival. In vivo, the cell adhesive environment is provided by extracellular matrix molecules, neighboring cell surfaces and soluble factors delivered either by tissue cells or by blood circulation. The exact molecular composition of the microenvironment of a cell is not properly understood. The nondefined molecular composition of “native” adhesive components hinders their application when defined culture conditions are necessary, as, for an example, growing human cells for further clinical application. Applying large, substrate-coating molecules as backbones for carrying specific adhesive peptide motifs provides a relatively cheap, reproducible, and chemically defined group of synthetic adhesion molecules. Here, we report on the design, synthesis, and testing of a novel cyclic RGD-containing coating material, which promotes initial attachment, spreading, survival, and proliferation of a number of different cell types. The potent adhesive polypeptide-brush, composed of poly[Lys(dl-Alam)] branched chain polypeptide (AK) and multiple copies of cyclic(arginyl-glycyl-aspartyl-d-phenylalanyl-cysteine) pentapeptide prevents anoikis and supports cell attachment in the absence of serum or other biological additives. The defined conditions for cell maintenance make this material a promising candidate for coating artificial cell substrates even for therapeutic applications.