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Download fileA Dual-Control Strategy by Phosphate Ions and Local Microviscosity for Tracking Adenosine Triphosphate Metabolism in Mitochondria and Cellular Activity Dynamically
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posted on 2021-10-28, 20:35 authored by Peng Zhang, Xinjie Guo, Jian Gao, Haihong Liu, Chenyang Wan, Jiajia Li, Qian Zhang, Yuqing Song, Caifeng DingAdenosine
triphosphate (ATP) acts as the main energy source for
growth and development in organisms, and the disorder reflects the
mitochondrial damage to a large extent. Therefore, an efficient tool
for the evaluation of the ATP metabolic level is important to track
mitochondrial health, providing an additional perspective for an in-depth
long-term study on living activities. Herein, a twisted intramolecular
charge transfer (TICT) framework is utilized to build up a sensitive
receptor, Mito-VP, with a negligible background to target mitochondrial
ATP metabolism by monitoring the phosphate ion (Pi) level upon ATP
hydrolysis under the overall consideration of the structural and functional
features of mitochondria. The responsive fluorescence could be lighted
on under the dual control of Pi and local microviscosity, and the
two steps of ATP hydrolysis could be captured through fluorescence.
In addition to the well-behaved mitochondrial targeting, the energy
metabolism at cellular and organism levels has been clarified via mitosis and zebrafish development, respectively.
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track mitochondrial healthbehaved mitochondrial targetingmain energy sourceatp metabolic levelatp hydrolysis couldresponsive fluorescence couldmitochondrial damageenergy metabolismvia two stepsterm studysensitive receptorphosphate ionsphosphate ionoverall considerationorganism levelsnegligible backgroundlocal microviscosityliving activitieslarge extentfunctional featuresefficient tooldisorder reflectsdepth longcontrol strategyadditional perspective