posted on 2021-11-16, 23:13authored byZhaojun Li, Quan Wang, Hongshu Jing, Xianghong Luo, Lianfang Du, Yourong Duan
Neovascularization
in plaque is the main driving factor for the
formation of vulnerable plaque. It is an effective strategy to alleviate
atherosclerosis by intervening neovascularization. Herein, we constructed
a cRGD peptide (cyclo(Arg–Gly–Asp–d-Phe–Cys))-modified
nanodelivery systems (RNPs) for targeted delivery of an antiangiogenesis
drug pigment epithelium-derived factor (PEDF), which is known as the
strongest endogenous angiogenesis inhibitor. PEDF RNPs have a suitable
size and good stability, which is conducive to passive target and
long-term blood circulation. In vitro cell uptake
study showed that cRGD on the surface of nanoparticles could bind
to the overexpressed ανβ3 integrin
in HUVECs, resulting in an increased uptake of PEDF RNPs by HUVECs. In vivo targeting experiments indicated that PEDF RNPs could
be effectively enriched in plaques by an EPR effect and a cRGD peptide.
In an apoE–/– mice model of advanced atherosclerosis,
we evaluated the therapeutic efficacy of PEDF RNPs by ultrasound imaging.
The result indicated that PEDF RNPs can inhibit intimal thickening.
Oil Red O (ORO) staining of isolated aorta showed that PEDF RNPs can
reduce the area of plaque, compared with free PEDF treatment. H&E
(hematoxylin–eosin) and Masson staining of the vessel cross
section further proved that PEDF RNPs had a nice curative effect.
CD31 staining suggested that the therapeutic effect of PEDF RNPs was
related to the inhibition of angiogenesis. This study provides a targeting
delivery system for inhibition of vasa vasorum in plaque, which has
the potential of clinical application for future atherosclerosis treatment.