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N-Methylation of Peptides on Selected Positions during the Elongation of the Peptide Chain in Solution Phase

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journal contribution
posted on 13.05.2005, 00:00 by M. Luisa Di Gioia, Antonella Leggio, Angelo Liguori
An efficient and general solution-phase method for the site-specific N-methylation of peptides has been developed. This novel procedure involves synthesis of N-nosyl protected peptides and their subsequent N-methylation with diazomethane. Its efficiency was proved by the successful synthesis of various hindered oligopeptides containing N-methyl amino acid residues with excellent yield and purity. The method is particularly attractive in that the adopted conditions do not cause any detectable racemization of the peptide stereocenters and the process does not require chromatographic purification of the methylated products. A further advantage is the compatibility of this methodology with Fmoc solution-phase peptide synthesis.