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De Novo Biosynthesis of Caffeic Acid from Glucose by Engineered Saccharomyces cerevisiae
journal contribution
posted on 2020-03-19, 12:03 authored by Yuanzi Li, Jiwei Mao, Quanli Liu, Xiaofei Song, Yuzhen Wu, Miao Cai, Haijin Xu, Mingqiang QiaoCaffeic acid is a plant phenolic
compound possessing extensive
pharmacological activities. Here, we identified that p-coumaric acid 3-hydroxylase from Arabidopsis thaliana was capable of hydroxylating p-coumaric acid to
form caffeic acid in Saccharomyces cerevisiae. Then,
we introduced a combined caffeic acid biosynthetic pathway into S. cerevisiae and obtained 0.183 mg L–1 caffeic acid from glucose. Next we improved the tyrosine biosynthesis
in S. cerevisiae by blocking the pathway flux
to aromatic alcohols and eliminating the tyrosine-induced feedback
inhibition resulting in caffeic acid production of 2.780 mg L–1. Finally, the medium was optimized, and the highest
caffeic acid production obtained was 11.432 mg L–1 in YPD medium containing 4% glucose. This study opens a route to
produce caffeic acid from glucose in S. cerevisiae and establishes a platform for the biosynthesis of caffeic acid
derived metabolites.