α‑Glucosidase Inhibitors from Cold-Pressed
Black Sesame (Sesamum indicum) Meal:
Characterization of New Furofuran Lignans, Kinetic Study, and In Vitro
Gastrointestinal Digestion
posted on 2023-12-05, 11:15authored byTitiruetai Doungwichitrkul, Thanakorn Damsud, Preecha Phuwapraisirisan
Black
sesame (Sesamum indicum) meal
is an agricultural waste obtained after oil extraction. It is used
as a key protein source in animal feed. Previous investigations have
indicated that its health benefits, such as antidiabetic activity,
are mainly due to its high lignan content. In the present study, we
applied α-glucosidase inhibitory guided isolation to identify
the active components responsible for the above claim. Twenty-nine
compounds, mostly lignans, were isolated and identified, of which
five (2–3, 12–13, and 28) were newly isolated. Of the isolated compounds, 20 and 21 were the most potent inhibitors, retarding
enzyme function in noncompetitive and uncompetitive manners. Structure–activity
relationship analysis suggested that the number of phenolic hydroxyl
groups in the structures was significantly related to the inhibitory
effect against α-glucosidase. A gastrointestinal digestion study
of the major lignan sesaminol triglucoside (STG, 9) suggested
that the transformation of dioxymethylene and glucoside moieties gradually
began in the late process, thus enhancing the α-glucosidase
inhibitory effect.