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Download fileYttrium Oxide as a Strongly Adsorbing but Nonquenching Surface for DNA Oligonucleotides
journal contribution
posted on 27.01.2020, 16:34 authored by Yangyang Chang, Biwu Liu, Zhicheng Huang, Yibo Liu, Meng Liu, Juewen LiuA large
number of nanomaterials can strongly adsorb DNA and quench
fluorescence, such as graphene oxide, gold nanoparticles, and most
metal oxides. On the other hand, noncationic nanomaterials that adsorb
DNA but cannot quench fluorescence are less known. These materials
are attractive for studying the mechanism of DNA-based surface reactions.
Y2O3 was found to have this property. Herein,
we used fluorescently labeled oligonucleotides as probes to study
the mechanism of DNA adsorption. The fluorescence was quenched at
low concentrations of Y2O3 and then recovered
and even enhanced with higher Y2O3 concentrations.
The reason was attributed to the intermolecular quenching by the DNA
bases of the neighboring strands. The fluorescence enhancement was
due to breaking of the intramolecular fluorophore/DNA interactions,
and the most enhancement was observed with a Cy3-labeled DNA. DNA
adsorption followed the Langmuir isotherm on Y2O3. Desorption experiments suggested that DNA was adsorbed through
the phosphate backbone, with FAM-G15 and FAM-C15 adsorbed more strongly than the other two DNA homopolymers. With
a high salt concentration, no fluorescence change was observed, suggesting
that the DNA adsorbed in a folded state reducing intermolecular quenching.
Overall, Y2O3 might be useful as a model surface
for investigating DNA hybridization on a surface.